Tyloses and phenolic deposits in xylem vessels impede water transport in low-lignin transgenic poplars: a study by cryo-fluorescence microscopy.
Identifieur interne : 003061 ( Main/Exploration ); précédent : 003060; suivant : 003062Tyloses and phenolic deposits in xylem vessels impede water transport in low-lignin transgenic poplars: a study by cryo-fluorescence microscopy.
Auteurs : Peter Kitin [Belgique] ; Steven L. Voelker ; Frederick C. Meinzer ; Hans Beeckman ; Steven H. Strauss ; Barbara LachenbruchSource :
- Plant physiology [ 1532-2548 ] ; 2010.
Descripteurs français
- KwdFr :
- Cellulose (analogues et dérivés), Cellulose (métabolisme), Coenzyme A ligases (métabolisme), Eau (métabolisme), Lignine (composition chimique), Microscopie confocale (MeSH), Microscopie de fluorescence (MeSH), Phénols (métabolisme), Populus (enzymologie), Populus (physiologie), Transport biologique (MeSH), Végétaux génétiquement modifiés (enzymologie), Végétaux génétiquement modifiés (physiologie), Xylème (anatomie et histologie), Xylème (métabolisme).
- MESH :
- analogues et dérivés : Cellulose.
- anatomie et histologie : Xylème.
- composition chimique : Lignine.
- enzymologie : Populus, Végétaux génétiquement modifiés.
- métabolisme : Cellulose, Coenzyme A ligases, Eau, Phénols, Xylème.
- physiologie : Populus, Végétaux génétiquement modifiés.
- Microscopie confocale, Microscopie de fluorescence, Transport biologique.
English descriptors
- KwdEn :
- Biological Transport (MeSH), Cellulose (analogs & derivatives), Cellulose (metabolism), Coenzyme A Ligases (metabolism), Lignin (chemistry), Microscopy, Confocal (MeSH), Microscopy, Fluorescence (MeSH), Phenols (metabolism), Plants, Genetically Modified (enzymology), Plants, Genetically Modified (physiology), Populus (enzymology), Populus (physiology), Water (metabolism), Xylem (anatomy & histology), Xylem (metabolism).
- MESH :
- chemical , analogs & derivatives : Cellulose.
- chemical , chemistry : Lignin.
- chemical , metabolism : Cellulose, Coenzyme A Ligases, Phenols, Water.
- anatomy & histology : Xylem.
- enzymology : Plants, Genetically Modified, Populus.
- metabolism : Xylem.
- physiology : Plants, Genetically Modified, Populus.
- Biological Transport, Microscopy, Confocal, Microscopy, Fluorescence.
Abstract
Of 14 transgenic poplar genotypes (Populus tremula × Populus alba) with antisense 4-coumarate:coenzyme A ligase that were grown in the field for 2 years, five that had substantial lignin reductions also had greatly reduced xylem-specific conductivity compared with that of control trees and those transgenic events with small reductions in lignin. For the two events with the lowest xylem lignin contents (greater than 40% reduction), we used light microscopy methods and acid fuchsin dye ascent studies to clarify what caused their reduced transport efficiency. A novel protocol involving dye stabilization and cryo-fluorescence microscopy enabled us to visualize the dye at the cellular level and to identify water-conducting pathways in the xylem. Cryo-fixed branch segments were planed in the frozen state on a sliding cryo-microtome and observed with an epifluorescence microscope equipped with a cryo-stage. We could then distinguish clearly between phenolic-occluded vessels, conductive (stain-filled) vessels, and nonconductive (water- or gas-filled) vessels. Low-lignin trees contained areas of nonconductive, brown xylem with patches of collapsed cells and patches of noncollapsed cells filled with phenolics. In contrast, phenolics and nonconductive vessels were rarely observed in normal colored wood of the low-lignin events. The results of cryo-fluorescence light microscopy were supported by observations with a confocal microscope after freeze drying of cryo-planed samples. Moreover, after extraction of the phenolics, confocal microscopy revealed that many of the vessels in the nonconductive xylem were blocked with tyloses. We conclude that reduced transport efficiency of the transgenic low-lignin xylem was largely caused by blockages from tyloses and phenolic deposits within vessels rather than by xylem collapse.
DOI: 10.1104/pp.110.156224
PubMed: 20639405
PubMed Central: PMC2949004
Affiliations:
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Voelker</name></author><author><name sortKey="Meinzer, Frederick C" sort="Meinzer, Frederick C" uniqKey="Meinzer F" first="Frederick C" last="Meinzer">Frederick C. Meinzer</name></author><author><name sortKey="Beeckman, Hans" sort="Beeckman, Hans" uniqKey="Beeckman H" first="Hans" last="Beeckman">Hans Beeckman</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author><author><name sortKey="Lachenbruch, Barbara" sort="Lachenbruch, Barbara" uniqKey="Lachenbruch B" first="Barbara" last="Lachenbruch">Barbara Lachenbruch</name></author></analytic><series><title level="j">Plant physiology</title><idno type="eISSN">1532-2548</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological Transport (MeSH)</term><term>Cellulose (analogs & derivatives)</term><term>Cellulose (metabolism)</term><term>Coenzyme A Ligases (metabolism)</term><term>Lignin (chemistry)</term><term>Microscopy, Confocal (MeSH)</term><term>Microscopy, Fluorescence (MeSH)</term><term>Phenols (metabolism)</term><term>Plants, Genetically Modified (enzymology)</term><term>Plants, Genetically Modified (physiology)</term><term>Populus (enzymology)</term><term>Populus (physiology)</term><term>Water (metabolism)</term><term>Xylem (anatomy & histology)</term><term>Xylem (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellulose (analogues et dérivés)</term><term>Cellulose (métabolisme)</term><term>Coenzyme A ligases (métabolisme)</term><term>Eau (métabolisme)</term><term>Lignine (composition chimique)</term><term>Microscopie confocale (MeSH)</term><term>Microscopie de fluorescence (MeSH)</term><term>Phénols (métabolisme)</term><term>Populus (enzymologie)</term><term>Populus (physiologie)</term><term>Transport biologique (MeSH)</term><term>Végétaux génétiquement modifiés (enzymologie)</term><term>Végétaux génétiquement modifiés (physiologie)</term><term>Xylème (anatomie et histologie)</term><term>Xylème (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Cellulose</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulose</term><term>Coenzyme A Ligases</term><term>Phenols</term><term>Water</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Cellulose</term></keywords><keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Xylème</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulose</term><term>Coenzyme A ligases</term><term>Eau</term><term>Phénols</term><term>Xylème</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Transport</term><term>Microscopy, Confocal</term><term>Microscopy, Fluorescence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Microscopie confocale</term><term>Microscopie de fluorescence</term><term>Transport biologique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Of 14 transgenic poplar genotypes (Populus tremula × Populus alba) with antisense 4-coumarate:coenzyme A ligase that were grown in the field for 2 years, five that had substantial lignin reductions also had greatly reduced xylem-specific conductivity compared with that of control trees and those transgenic events with small reductions in lignin. For the two events with the lowest xylem lignin contents (greater than 40% reduction), we used light microscopy methods and acid fuchsin dye ascent studies to clarify what caused their reduced transport efficiency. A novel protocol involving dye stabilization and cryo-fluorescence microscopy enabled us to visualize the dye at the cellular level and to identify water-conducting pathways in the xylem. Cryo-fixed branch segments were planed in the frozen state on a sliding cryo-microtome and observed with an epifluorescence microscope equipped with a cryo-stage. We could then distinguish clearly between phenolic-occluded vessels, conductive (stain-filled) vessels, and nonconductive (water- or gas-filled) vessels. Low-lignin trees contained areas of nonconductive, brown xylem with patches of collapsed cells and patches of noncollapsed cells filled with phenolics. In contrast, phenolics and nonconductive vessels were rarely observed in normal colored wood of the low-lignin events. The results of cryo-fluorescence light microscopy were supported by observations with a confocal microscope after freeze drying of cryo-planed samples. Moreover, after extraction of the phenolics, confocal microscopy revealed that many of the vessels in the nonconductive xylem were blocked with tyloses. We conclude that reduced transport efficiency of the transgenic low-lignin xylem was largely caused by blockages from tyloses and phenolic deposits within vessels rather than by xylem collapse.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20639405</PMID><DateCompleted><Year>2011</Year><Month>01</Month><Day>03</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>22</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1532-2548</ISSN><JournalIssue CitedMedium="Internet"><Volume>154</Volume><Issue>2</Issue><PubDate><Year>2010</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Tyloses and phenolic deposits in xylem vessels impede water transport in low-lignin transgenic poplars: a study by cryo-fluorescence microscopy.</ArticleTitle><Pagination><MedlinePgn>887-98</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1104/pp.110.156224</ELocationID><Abstract><AbstractText>Of 14 transgenic poplar genotypes (Populus tremula × Populus alba) with antisense 4-coumarate:coenzyme A ligase that were grown in the field for 2 years, five that had substantial lignin reductions also had greatly reduced xylem-specific conductivity compared with that of control trees and those transgenic events with small reductions in lignin. For the two events with the lowest xylem lignin contents (greater than 40% reduction), we used light microscopy methods and acid fuchsin dye ascent studies to clarify what caused their reduced transport efficiency. A novel protocol involving dye stabilization and cryo-fluorescence microscopy enabled us to visualize the dye at the cellular level and to identify water-conducting pathways in the xylem. Cryo-fixed branch segments were planed in the frozen state on a sliding cryo-microtome and observed with an epifluorescence microscope equipped with a cryo-stage. We could then distinguish clearly between phenolic-occluded vessels, conductive (stain-filled) vessels, and nonconductive (water- or gas-filled) vessels. Low-lignin trees contained areas of nonconductive, brown xylem with patches of collapsed cells and patches of noncollapsed cells filled with phenolics. In contrast, phenolics and nonconductive vessels were rarely observed in normal colored wood of the low-lignin events. The results of cryo-fluorescence light microscopy were supported by observations with a confocal microscope after freeze drying of cryo-planed samples. Moreover, after extraction of the phenolics, confocal microscopy revealed that many of the vessels in the nonconductive xylem were blocked with tyloses. We conclude that reduced transport efficiency of the transgenic low-lignin xylem was largely caused by blockages from tyloses and phenolic deposits within vessels rather than by xylem collapse.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kitin</LastName><ForeName>Peter</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Laboratory for Wood Biology and Xylarium, Royal Museum for Central Africa, Tervuren, Belgium. peter.kitin@oregonstate.edu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Voelker</LastName><ForeName>Steven L</ForeName><Initials>SL</Initials></Author><Author ValidYN="Y"><LastName>Meinzer</LastName><ForeName>Frederick C</ForeName><Initials>FC</Initials></Author><Author ValidYN="Y"><LastName>Beeckman</LastName><ForeName>Hans</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Strauss</LastName><ForeName>Steven H</ForeName><Initials>SH</Initials></Author><Author ValidYN="Y"><LastName>Lachenbruch</LastName><ForeName>Barbara</ForeName><Initials>B</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>1S10RR107903-01</GrantID><Acronym>RR</Acronym><Agency>NCRR NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>07</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010636">Phenols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C017962">Tyloses</NameOfSubstance></Chemical><Chemical><RegistryNumber>059QF0KO0R</RegistryNumber><NameOfSubstance UI="D014867">Water</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 6.2.1.-</RegistryNumber><NameOfSubstance UI="D003066">Coenzyme A Ligases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001692" MajorTopicYN="N">Biological Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="Y">analogs & derivatives</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003066" MajorTopicYN="N">Coenzyme A Ligases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008856" MajorTopicYN="N">Microscopy, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010636" MajorTopicYN="N">Phenols</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014867" MajorTopicYN="N">Water</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>7</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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Meinzer</name><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name><name sortKey="Voelker, Steven L" sort="Voelker, Steven L" uniqKey="Voelker S" first="Steven L" last="Voelker">Steven L. Voelker</name></noCountry><country name="Belgique"><noRegion><name sortKey="Kitin, Peter" sort="Kitin, Peter" uniqKey="Kitin P" first="Peter" last="Kitin">Peter Kitin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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